The Spectrum of Major Seed Storage Genes and Proteins in Oats (Avena sativa)
نویسنده
چکیده
BACKGROUND The oat seed storage proteins are mainly composed of two classes: the globulins and avenins. Among the major cereals, the globulins are the major seed protein class in rice and oats, and along with the higher protein content of oats is the basis for the relative higher nutrition content in oats compared to the other cereals. The second major class of oat seed proteins is the avenins; also classified as prolamins - seed proteins high in proline and glutamine amino acids. The prolamins are associated with celiac disease, an autoimmune disorder of the gastrointestinal tract. In spite of their importance, neither the oat globulins nor the avenins have been completely analyzed and described for any single germplasm. RESULTS Using available EST resources for a single hexaploid oat cultivar, the spectrum of avenin and globulin sequences are described for the gene coding regions and the derived protein sequences. The nine unique avenin sequences are suggested to be divided into 3-4 distinct subclasses distributed in the hexaploid genome. The globulins from the same germplasm include 24 distinct sequences. Variation in globulin size results mainly from a glutamine-rich domain, similar to as in the avenins, and to variation in the C-terminal sequence domain. Two globulin genes have premature stop codons that shorten the resulting polypeptides by 9 and 17 amino acids, and eight of the globulin sequences form a branch of the globulins not previously reported. CONCLUSIONS A more complete description of the major oat seed proteins should allow a more thorough analysis of their contributions to those oat seed characteristics related to nutritional value, evolutionary history, and celiac disease association.
منابع مشابه
Subgenome‐specific assembly of vitamin E biosynthesis genes and expression patterns during seed development provide insight into the evolution of oat genome
Vitamin E is essential for humans and thus must be a component of a healthy diet. Among the cereal grains, hexaploid oats (Avena sativa L.) have high vitamin E content. To date, no gene sequences in the vitamin E biosynthesis pathway have been reported for oats. Using deep sequencing and orthology-guided assembly, coding sequences of genes for each step in vitamin E synthesis in oats were recon...
متن کاملA STUDY ON ULTRASTRUCTURAL ASPECTS OF SEED DORMANCY IN WILD OATS
The structural differences between dormant and nondormant seeds of wild oats (Avena ludoviciana Durieu) were studied by light and transmission electron microscopy. Radial elongation of scutellum epithelial cells were observed in nondormant seeds after imbibition, compared with dormant seeds in which cells were completely compressed. Ultrastructural differences for example, dilution of cytopla...
متن کاملIdentification of Oats (Avena sativa L.) Cultivars by Seed and Seedling Protein Electrophoresis
Soluble proteins were extracted from seed and seedlings of eleven oats cultivars to analyze the protein profile through SDS PAGE method. In seed protein electrophoresis totally 14 bands were observed, in these six bands were common to all cultivars. Based on the seed protein banding pattern two of these cultivars were differentiated easily. The unique band at Rf value 0.151 was common for all c...
متن کاملMOLECULAR ANALYSIS OF FATTY ACID PEROXYGENASE INVOLVED IN THE BIOSYNTHESIS OF EPOXY FATTY ACIDS IN OATS (Avena sativa)
متن کامل
In vitro antioxidant activity and inhibitory effect, on oleic acid-induced hepatic steatosis, of fractions and subfractions from oat (Avena sativa L.) ethanol extract
Oats (Avena sativa L.) were extracted with 80% aqueous ethanol and the extract was successively isolated by liquid–liquid partition to yield n-hexane, ethyl acetate, n-butanol and water layers. Among them, the ethyl acetate (EA) layer exhibited the highest total phenolic content (TPC), the strongest DPPH radicalscavenging activity and an inhibitory effect on an oleic acid-induced (OA-induced) f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 9 شماره
صفحات -
تاریخ انتشار 2014